fluorescent band intensity Search Results


li cor odyssey imaging system  (LI-COR)
99
LI-COR li cor odyssey imaging system
Li Cor Odyssey Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna band fluorescent intensities  (Thermo Fisher)
99
Thermo Fisher dna band fluorescent intensities
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Dna Band Fluorescent Intensities, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent intensity values  (Molecular Biosciences Inc)
90
Molecular Biosciences Inc fluorescent intensity values
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Fluorescent Intensity Values, supplied by Molecular Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent band intensity  (GE Healthcare)
95
GE Healthcare fluorescent band intensity
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Fluorescent Band Intensity, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyan fluorescent protein intensity  (IDEX)
90
IDEX cyan fluorescent protein intensity
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Cyan Fluorescent Protein Intensity, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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odyssey vis spectrophotometer software  (LI-COR)
86
LI-COR odyssey vis spectrophotometer software
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Odyssey Vis Spectrophotometer Software, supplied by LI-COR, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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storm 800 fluorescence phosphorimager  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc storm 800 fluorescence phosphorimager
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Storm 800 Fluorescence Phosphorimager, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence scanner  (LI-COR)
99
LI-COR fluorescence scanner
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Fluorescence Scanner, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescence scanner - by Bioz Stars, 2026-04
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band  (LI-COR)
99
LI-COR band
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Band, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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band - by Bioz Stars, 2026-04
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sds page gels  (Bio-Rad)
98
Bio-Rad sds page gels
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Sds Page Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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snarf-1 fluorescence bands  (OriginLab corp)
90
OriginLab corp snarf-1 fluorescence bands
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Snarf 1 Fluorescence Bands, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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li cor image studio lite software  (LI-COR)
86
LI-COR li cor image studio lite software
PCR amplification of <t>DNA</t> containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures <t>by</t> <t>capillary</t> electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.
Li Cor Image Studio Lite Software, supplied by LI-COR, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PCR amplification of DNA containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures by capillary electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.

Journal:

Article Title: Reduction of Stability of Arabidopsis Genomic and Transgenic DNA-Repeat Sequences (Microsatellites) by Inactivation of AtMSH2 Mismatch-Repair Function 1

doi: 10.1104/pp.103.023952

Figure Lengend Snippet: PCR amplification of DNA containing each of nine different microsatellites from each progeny plant with one of each locus-specific primer pairs, analysis of product mixtures by capillary electrophoresis, detection of fluorescent products, and generation of electropherograms were as described under “Materials and Methods.” A, Electropherograms showing all PCR products; one of each primer pair was labeled with FAM or HEX fluorescent dye. B, Representative electropherogram patterns for locus NGA1107. Apparent absolute size of primary wild-type (wt) product band is 317 bp. Shown also are products where both alleles showed an increase of one repeat unit, increasing apparent product length to 319 bp (+2), where one allele maintained wild-type length and one showed a loss of one repeat [wt & (-2)], and where one allele remained wild type and one showed a shift of two repeats, decreasing apparent length to 313 bp [wt & (-4)]. Arrows indicate position of 317-bp (wild-type) product. C, Patterns for reconstruction mixtures. DNA from plants homozygous (pure) for alleles at locus NGA1107 encoding 313-bp (black arrow) or 315-bp (gray arrow) products or mixtures of the two at indicated ratios (concentrations of DNAs measured before PCR by staining with PicoGreen [Molecular Probes, Eugene, OR]) were analyzed as described above. Note that the primary criterion for scoring a pattern as reflecting a mixture of two different length products—second peak from right greater than a significantly large first peak—is met only for 1:1 and 2:1 mixtures.

Article Snippet: Up to nine locus-specific PCR reaction products were diluted as empirically found appropriate for subsequent analysis, mixed, and analyzed by capillary electrophoresis and measurement of DNA-band fluorescent intensities, using the ABI Prism 3100 Genetic Analyzer and associated software (Applied Biosystems, Palo Alto, CA).

Techniques: Amplification, Electrophoresis, Labeling, Staining